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Fig. 3 Heterologous cell interactions in the axial vascular area in the trunk of control embryos. A,B: Contact zone of dorsal aorta-endothelial cells (ECs) with the hypochord (A) and (B) of posterior cardinal vein-ECs with the endoderm. In both A and B, the cells contact each other by means of cellular extensions (arrows) approaching the opposed cell to a minimal distance. Arrowhead in A, gap junction. C: Between ECs and red blood cell precursors, deposits of extracellular material (arrows) are present at irregular distances. ED, endoderm; H, hypochord; N, notochord sheath; R, red blood cell precursor. Scale bars = 500 nm in A, 2 μm in B, 200 nm in C.