Fig. 8

Fig. 8 Foxd3 negatively regulates mitfa. (A–D) Lateral views of mitfa in situ hybridizations of 32 hpf embryos. (B, D) There is an increase in the number of mitfa-positive melanoblasts in hdac1^col/foxd3 mutant/morphant embryos as compared to uninjected hdac1^col mutants. (A, C) In wild-type/foxd3 morphant embryos mitfa expression in melanoblasts is more robust than uninjected controls. (E–H) Lateral views of c-kit in situ hybridizations in 32 hpf embryos. (F, H) There is no rescue of c-kit expression in melanoblasts in hdac1^col/foxd3 mutant morphants when compared to uninjected hdac1^col mutants at 32 hpf. (E, G) Qualitatively, there are fewer c-kit positive melanoblasts in foxd3 morpholino treated wild-type embryos as compared to uninjected wild-type embryos. (E–H) In contrast to melanoblast expression, there is no difference in c-kit expression in the post anal region between wild-type, hdac1^col mutants, wt/foxd3 wild-type/morphants and hdac1^col/foxd3 mutant/morphant embryos (arrows).