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Fig. 2 cdx1b antisense MO knockdown analyses. cdx1b 24 hpf (A-F) and 48 hpf (M,N) zebrafish morphants; cdx1b-4mm-MO-injected 24 hpf (G-I) and 48 hpf (O,P) embryos; wild-type 24 hpf (J-L) and 48 hpf (Q,R) embryos. Green fluorescence was not detected in cdx1b MO and CMV-cdx1b-mo-GFP co-injected (T) 27 hpf embryos, whereas bright green fluorescence was detected in cdx1b-4mm MO and CMV-cdx1b-mo-GFP co-injected embryos (S). The expression patterns of shh were compared in 28 hpf wild type (U,V) and morphants (W,X). Scale bars: 100 μm. a, atrium; bp, basal plate midbrain; fp, floor plate; hy, hypothalamus; v, ventricle; wt, wild type; zli, zona limitans intrathalamica.