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Fig. 1 chch inhibition results in axial and somite defects. The effect of inhibiting chch was examined using a morpholino and a dominant-negative construct. Microinjection of chch-ATGMO results in a broad, misshapen notochord (arrow) and misshapen somites and a shortened axis (B). Microinjection of chch-EnR, results in a similar phenotype as the morpholino, a broad, misshapen notochord, and enlarged and misshapen somites formed (C). chch mRNA overexpression produces embryos that are wild-type in appearance (D). Arrowheads point to the notochord. All embryos are 24 hpf.
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Full text @ BMC Dev. Biol.