ZFIN Image: Sato et al., 2005, Fig. 3

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Figure Caption

Fig. 3 Double fluorescent labeling of transgene products and endogenous mRNA in the OE of OMP:YFP and TRPC2:Venus transgenic fish.A-C, The expression of zOMP mRNA (magenta; A, C) and YFP protein (green; B, C) in the OMP:YFP line. D-F, The expression of zTRPC2 mRNA (magenta; D, F) and Venus protein (green; E, F) in the TRPC2:Venus line. All photographs represent stacked images of optical sections. Asterisks denote the position of somata labeled with anti-GFP. In the OMP:YFP line, YFP was expressed in cells that express zOMP mRNA in the deep layer of the OE (asterisks; B, C). In the TRPC2:Venus line, Venus was expressed in zTRPC2-positive cells in the superficial layer (asterisks; E, F). However, some zTRPC2-positive cells appeared to be unlabeled (arrows; F). The apical surface of the OE is at the top. Scale bar, 50 μm.

Acknowledgments

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