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Fig. S6 AML1-ETO suppresses the erythroid and monocytic cell fates, and promotes the granulocytic cell fate in chordin morphants. (A) In situ hybridization of gata1, mpo and l-plastin in wild-type and AML1-ETO transgenic embryos. All of the embryos have been injected with 50 mM chordin antisense morpholino oligonucleotides (Open Biosystems) and subjected to the same heat treatment. The expanded pool of blood cells in the chordin morphants expresses gata1 and l-plastin (A, upper panels), whereas induced expression of AML1-ETO downregulates both gata1 and l-plastin but upregulates mpo (A, lower panels). The embryos were at 22 hpf for gata1 staining and 31 hpf for mpo and l-plastin staining. (B,C) Cross sections of chordin morphants stained with l-plastin probes. While l-plastin+ cells are often seen in the wild-type embryos, these cells are not seen in the AML1-ETO transgenic embryos.