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Fig. 4 en1b+ and evx1+ interneurons increase in cyclopamine-treated embryos. A-D: Lateral whole-mount views of subtype-specific postmitotic marker are shown with rostral to the left at 24 hours postfertilization (hpf). A-F: en1b+ (A,C,E) and pax2.1+ (B,D,F) interneurons are increased in cyclopamine-treated embryos. Error bars represent SEM. G,H: Representative sections of green fluorescent protein (GFP) expression in Tg(elavl3:EGFP)zf8 embryos are shown. There is no change in the number of Hu-positive neurons after cyclopamine treatment.
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