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Fig. S1 tbx16 RNA is expressed in DFCs during gastrulation. At 60% epiboly (A) and 80% epiboly (B) RNA in situ hybridization detected tbx16 expression in prechordal plate, paraxial mesoderm, margin cells and DFCs (black arrows). At 4–6 SS (C) tbx16 was strongly expressed in the tailbud (white arrow), making it difficult to determine whether tbx16 was also expressed in KV cells.
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Reprinted from Developmental Biology, 310(2), Amack, J.D., Wang, X., and Yost, H.J., Two T-box genes play independent and cooperative roles to regulate morphogenesis of ciliated Kupffer's vesicle in zebrafish, 196-210, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.