ZFIN Image: Walker et al., 2006, Fig. S4

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Figure Caption

Fig. S4 furinA genetically interacts with edn1. Offspring of a furinA^tg419/+ × edn1^tf216b/+ cross were raised to adulthood. furinA^/+;edn1^/+ double heterozygote carriers were identified by PCR genotyping finclips. Double heterozygote carriers were mated and subsequent progeny were initially sorted into mutant and wild-type classes based on an open-mouth phenotype and ruffled fin phenotype. The heads of embryos were fixed and stained with Alcian green at 6 dpf and genomic DNA for genotyping was prepared from the tails. Loss of a single copy of edn1 in furinA mutants result in a high penetrance of severe reductions in the palatoquadrate (pq) and ceratohyal (ch) cartilages. edn1;furinA double mutants resemble edn1 single mutants. (A) wild-type, (B) furinA^tg419, (C) furinA^tg419;edn1^tf216b/+, (D) edn1^tf216b, (E) furinA^tg419;edn1^tf216b.

Acknowledgments

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Reprinted from Developmental Biology, 295(1), Walker, M.B., Miller, C.T., Talbot, J.C., Stock, D.W., and Kimmel, C.B., Zebrafish furin mutants reveal intricacies in regulating Endothelin1 signaling in craniofacial patterning, 194-205, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.