Fig. 7

Fig. 7 Zebrafish Fmi1a and Fmi1b proteins co-operate to control cell movement. (A–C, I, J) 10-somite embryos. (E, F, H, L–N) 3-somite embryos. In all dorsal views, anterior is to the top. In all lateral views, dorsal is to the right. Markers are as indicated, px is pax2.1, k is krox20, papc is paraxial protocadherin. (A–C) F1b-GPS1 morphants exhibit undulation of neuroepithelium and tailbud. (D and K) RT-PCR analysis. Titration of F1b-GPS1 (D) and F1a-LamG2 (K) morpholino. CoMo is F1b-GPS or F1a-LamG2 morpholino but with four nucleotide changes. Molecular markers (bp) are indicated to left of gel. (D) 3 μg/ml F1b-GPS1 morpholino generates approximately 65% wild-type and 35% mis-spliced product. P1/P2 indicates primer pair used in PCR (see Fig. 2A). (K) 5 μg/ml F1a-LamG2 morpholino generates approximately 40% wild-type and 60% mis-spliced product. P2/P3 indicates primer pair used in PCR (see Fig. 2G). (E–I) Double morphant embryos. (E) Injection of 2.4 μg/ml and 3.5 μg/ml CoMos for F1a-GPS1 and F1b-GPS1, respectively. (F) Injection of 2.4 μg/ml F1a-GPS1 and 3.5 μg/ml F1b-GPS1. (G) F1a-GPS1 (3 μg/ml) and F1b-GPS1 (3.5 μg/ml) morphant. (H) F1a-GPS1 (4 μg/ml) and F1b-GPS1 (4 μg/ml) morphant. Black arrowhead: mid-hindbrain pax2 expression; white arrow: papc expression; black arrow: pronephric pax2 expression. (I) F1a-GPS1 (2.4 μg/ml) and F1b-GPS1 (3.5 μg/ml) morphant. View of head, arrow indicates pax2.1 expression in optic stalk. (J) 9-somite embryo. (L–M) Double morphant embryo, (L) F1a-GPS1 (2.4 μg/ml) and F1a-LamG2 (4.5 μg/ml). (M) F1a-LamG2 (4.5 μg/ml) and F1b-GPS1 (3.5 μg/ml).