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Fig. 1 Structural characterization of the zebrafish ALP subfamily. (A) Gene structures of zebrafish elfin, mystique, alp, alp-like and ril are shown. Overall alp family genes demonstrate relative conserved intron/exon structures. For all five genes the isolated, largest splice variant are depicted, for Elfin in addition a verified shorter spliceform is shown (CN505168). Other splice forms have been described in different organisms for Alp, Mystique and Ril but not for Elfin (Bashirova et al., 1998, Andersen et al., 2004 and Loughran et al., 2005). Numbers indicated correspond to the amino acid (Aa) sequences. (B) Alignment of Aa sequences of the PDZ- (shaded in blue) and LIM-domains (shaded in yellow) for the zebrafish ALP family. Fully conserved residues are marked in black and partial conservation is indicated in grey. (C) Phylogenetic analysis of zebrafish alp genes. Shown is a phylogenetic tree of the zebrafish ALP subfamily including human and mouse sequences. The unrooted tree was constructed by neighbour-joining analysis based on the amino acid sequences.
Reprinted from Gene expression patterns : GEP, 7(3), Te Velthuis, A.J., Ott, E.B., Marques, I.J., and Bagowski, C.P., Gene expression patterns of the ALP family during zebrafish development, 297-305, Copyright (2007) with permission from Elsevier. Full text @ Gene Expr. Patterns