Fig. 4

Fig. 4 Splice junction targeted anti-blimp-1 MOs effectively block splicing of blimp-1 pre-mRNA. (A) RT-PCR of mRNA extracted from wild-type embryos and blimp-1 morphants, showing the expected 783 bp band in the wild-type lanes and an approximately 3 kb band in those of the morphants. The primer pair used for the PCR reaction amplifies across exons 1-5 (see Materials and methods). (B) Diagram illustrating the target site of the MO, at the junction between exon 2 and intron 2. The last codon of exon 2 and the first codon of exon 3, together with their corresponding amino acid, are indicated. The premature stop codon in the mis-spliced blimp-1 mRNA is highlighted in red.