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Fig. S1 Morphologic and Molecular Changes Caused by tob1a Overexpression and Knockdown (A) Embryos injected with 400 pg of tob1a mRNA were ventralized to variable degrees at 24 hpf. Note the posterior progression of head deletion. (B) Embryos injected with 15 ng of tob1a-MO were dorsalized to variable degrees at 24 hpf. Note the progressive shortening of tail and yolk extension. (C) Embryos injected with 15 ng of tob1a-cMO, a control morpholino that has a sequence identical to tob1a-MO except 6 mismatch nucleotides, did not produce visible phenotypes. (D-E) Ratios of embryos with altered marker gene expression, following injection with tob1a mRNA (D) or tob1a-MO (E). chordin, goosecoid and otx2 are dorsal markers and the others are ventral markers. Downregulation and upregulation were indicated by upward and downward arrowheads in (E), respectively. Corresponding pictures were shown in Figure 2.
Reprinted from Developmental Cell, 11(2), Xiong, B., Rui, Y., Zhang, M., Shi, K., Jia, S., Tian, T., Yin, K., Huang, H., Lin, S., Zhao, X., Chen, Y., Chen, Y.G., Lin, S.C., and Meng, A., Tob1 controls dorsal development of zebrafish embryos by antagonizing maternal beta-catenin transcriptional activity, 225-238, Copyright (2006) with permission from Elsevier. Full text @ Dev. Cell