Fig. 2

Fig. 2 Elimination of β-catenin signaling induces the ectopic expression of dorsal genes around the germ-ring. At 75% epiboly, wild-type embryos have characteristic patterns of chd (A) and nog1 (F) expression, whereas ich embryos do not express these genes (B and G). Injection of βcat1MO (MO1) into ich embryos induces expression of chd (H) and nog1 (H) around the germ-ring, but βcat2MO (MO2) has no effect (D and I). Co-injection of the two MOs into ich embryos causes somewhat increased expression of chd (E) and nog1 (J) around the germ-ring. Concentrations of MOs injected were 3 mM for the single MO1 or MO2 injections and 3 mM each for the double MO injection. Wild-type embryos are shown in dorsal view (A and F) and all other embryos are shown in lateral views. Expression (or lack of) was identical for all embryos for each treatment (>16 in each case) except for (I) in which 1/19 embryos showed very weak expression.