Fig. 6

Fig. 6 Effect of Fyn^K299M on the ability of blastomeres to participate in epiboly. Embryos were collected at the 16-cell stage and single blastomeres were injected with a mixture of calcium green-dextran and mRNA (4.5 pg). The embryos were cultured at 28°C and examined by confocal fluorescence microscopy at 5, 7, and 9 hpf. The embryo shown in the upper panels was injected with c-Fyn mRNA and the fluorescent cells migrated toward the vegetal pole with the advancing blastoderm. The embryos shown in the middle and lower panels were injected with Fyn^K299M mRNA and in these cases, the fluorescent cells remained near the animal pole.