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Figure Caption
Fig. 4 Morphology of embryos arrested following FynK299M mRNA or GST-Fyn-SH2 injection. Zygotes were injected with 230–250 pg of mRNA encoding c-Fyn (A–C) or FynK299M (D–F), or a mixture of c-Fyn (230 pg) and of FynK299M (270 pg), then incubated in embryo medium at 28°C. Zygotes injected with the GST-Fyn-SH2 fusion protein (1.3 pmol) also ceased development at the onset of epiboly (J, K, L). They were examined at 2.5 hpf (A, D, G, J), 4.0 hpf (B, E, H, K), and at 5.25 hpf (C, F, I, L). Magnification is indicated by the bar in panel A which indicates 100 μm.
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Reprinted from Developmental Biology, 285(2), Sharma, D., Holets, L., Zhang, X., and Kinsey, W.H., Role of Fyn kinase in signaling associated with epiboly during zebrafish development, 462-476, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.