ZFIN Image: Sharma et al., 2005, Fig. 2

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Figure Caption

Fig. 2 Expression of Fyn kinase following mRNA injection. The level of Fyn protein expression was monitored by Western blot analysis using an anti-peptide antibody specific for Fyn kinase (Fyn-3 antibody from Santa Cruz Biotechnology, Santa Cruz, CA). Membrane fractions were prepared and Western blots were performed from groups of 20 embryos as previously described (Wu and Kinsey, 2003). Samples containing identical amounts of protein from control, uninjected embryos (-), from embryos injected with 230 pg of Fyn^K299M (dn-Fyn), or c-Fyn mRNA (c-Fyn) were collected at 3.3 hpf (high stage, A), 3.8 hpf (sphere stage, B), or at 4.5 hpf when controls were at 25% epiboly (C).

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Acknowledgments

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Reprinted from Developmental Biology, 285(2), Sharma, D., Holets, L., Zhang, X., and Kinsey, W.H., Role of Fyn kinase in signaling associated with epiboly during zebrafish development, 462-476, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.