Fig. 7

Fig. 7 Effects of grl antisense morpholino injection on the interrenal tissue and its neighboring vasculature. Single confocal sections showing the interrenal tissues as detected by 3β-Hsd activity staining (left panel: A, D, G, J), and the neighboring ECs as labeled by green fluorescence (middle panel: B, E, H, K), of 56 hpf Tg(fli1:EGFP)^Y1 embryos injected with STD-MO (A–C, G–I) and grlMO (D–F, J–L), respectively. The merged images of 3β-Hsd activity staining and GFP are shown in the right panels C, F, I and L. Panels A–F are dorsal views with anterior oriented to the top, while panels G–L are dorsolateral views with anterior to the right. The disruption of dorsal aorta through injection of grl morpholino does not significantly perturb the interrenal convergence. DA, dorsal aorta; PCV, posterior cardinal vein. Red arrows indicate interrenal tissues, while yellow arrowheads indicate glomeruli. Scale bar, 50 μM.