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Fig. S1 pit1 morphant eya1 mutants lack expression of all hormone genes. All panels show ventral views on the head region of embryos at 32 hpf, after in situ hybridization with probes indicated in lower right corners. (A, E, I) Wild type; (B, F, J) wild type injected with pit1 antisense morpholino oligonucleotide (pit1 MO); (C, G, K) eya1 mutant; (D, H, L) eya1 mutant injected with pit1 MO. Arrows in panels (E, G, H) point to pomc-positive cells in the arcuate nucleus. Scale bars are 50 μm.
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Reprinted from Developmental Biology, 292(1), Nica, G., Herzog, W., Sonntag, C., Nowak, M., Schwarz, H., Zapata, A.G., Hammerschmidt, M., Eya1 is required for lineage-specific differentiation, but not for cell survival in the zebrafish adenohypophysis, 189-204, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.