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Fig. 5 Expression of kir7.1 genes. RT-PCR was performed with RNA samples isolated from the indicated tissues of adult fish. M indicates the molecular size marker. Total RNAs were reverse-transcribed with oligo d(T)18 primer, and the resultant cDNAs were amplified by PCR using specific primers for kir7.1 and β-actin (positive control). A; cichlid. B; zebrafish. C; pufferfish.
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Reprinted from Gene, 399(1), Watanabe, M., Hiraide, K., and Okada, N., Functional diversification of kir7.1 in cichlids accelerated by gene duplication, 46-52, Copyright (2007) with permission from Elsevier. Full text @ Gene