ZFIN Image: Dickmeis et al., 2007, Fig. 2

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Figure Caption

Fig. 2 Attenuated Cell Cycle Rhythms Are Not Due to Changes in Circadian Clock Gene Expression or Disturbed Pineal Melatonin Output (A) Circadian expression of clock genes in mutant larvae of both weak and strong alleles of rx3. Representative RNase protection assay results for per4, clock1, and μ-actin expression using RNA harvested at the indicated ZTs. “t” represents a tRNA negative control. (B) Circadian expression of aanat2 in strong rx3 mutants. Whole-mount in situ hybridization for aanat2 in strong rx3 mutants (“strong”) and wild-type siblings (“wildtype”) at four circadian time points (6 dpf). Images show dorsal views of the pineal gland (arrow), rostral up. (C) Quantification of BrdU labeled nuclei in luzindole treated wild-type larvae. Means of BrdU-positive nuclei determined as described in Figure 1 are shown at four circadian time points on 6 dpf for luzindole-treated larvae (+, blue) and control larvae (-, red). Error bars show 95% confidence interval of the mean.

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Expression / Labeling details

Acknowledgments

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