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Fig. 3 Nascent and Mature Transcripts Visualized by In Situ Hybridisation. Fluorescent staining by ISH for her1 (top left), her7 (top middle), and deltaC (top right), using tyramide chemistry (green), reveals nuclear dots corresponding to nascent transcripts and cytoplasmic signal corresponding to mature mRNA. The bottom panel is a magnified detail of the top left panel. The images are confocal optical sections of flat-mounted specimens, counterstained for DNA with TOPRO3 (blue false colour). Red staining shows myoD expression by dual ISH.
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