Fig. 6

Fig. 6 Expression of tbx5 and fibin in pectoral fin buds during zebrafish embryonic development. The expression of tbx5 (a–e) and fibin (f–j) was examined in presumptive pectoral fin bud regions during different developmental stages (13∼30 hpf) by whole mount in situ hybridization. The expression of tbx5 and fibin in presumptive pectoral fin bud regions at 22 hpf was also examined at transverse sections (16 μm) (k, l). The expression of pax2.1 was examined in embryos at 14 hpf by whole mount in situ hybridization (m). The expression of pax2.1 and fibin was examined in embryos at 14 hpf by double whole mount in situ hybridization (n). The expression of tbx5 and fibin was also examined in presumptive pectoral fin bud regions at 24 hpf by double whole mount in situ hybridization (o). Arrowheads and arrows indicate the expression of tbx5 and fibin in presumptive pectoral fin bud regions, respectively. S1, S2 and S3 indicate the locations of somite 1, somite 2 and somite 3, respectively.