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Fig. S2 Cartilage defects and absence of ectodermal gcm2 expression in 1aATGMO-injected animals. (A, D, E and G) Wildtype animals. (B, C, F and H) 1aATGMO-injected animals. (A and B) Dissected ventral cartilage elements stained with alcian blue at 4 dpf. (C and D) Ceratobranchial cartilage elements stained with aclian blue at 4 dpf. (E and F) Dissected neurocranium from alcian blue-stained 4 dpf larvae. (G and H) Lateral views of gcm2 expression within pharyngeal domain at 48 hpf. Inserts represent enlarged views of boxed regions. Asterisks mark gcm2 expression within ectodermal lining of branchial arches. Asterisk in H marks persistent gcm2 expression within 1aATGMO-injected embryo. Abbreviations: abc, anterior basicranial commissure; cb1–5, ceratobranchial arch 1–5; ch, ceratohyal; ep, ethmoid plate; hs, hyosymplectic; m, Meckel’s cartilage; no, notochord; pc, parachordal; pq, palatoquadrate; tr, trabecula. Scale bars: 100 μm.
Reprinted from Mechanisms of Development, 123(12), Hall, C., Flores, M.V., Murison, G., Crosier, K., and Crosier, P., An essential role for zebrafish Fgfrl1 during gill cartilage development, 925-940, Copyright (2006) with permission from Elsevier. Full text @ Mech. Dev.