ZFIN Image: Kinna et al., 2006, Fig. 5

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Figure Caption

Fig. 5 crim1 morphants have an expanded intermediate cell mass. (A–G′) Wholemount RNA in situ hybridisation of 24 hpf embryos injected with 5 ng crim1A-MO (B, E, F, H) and uninjected controls (A, C, E, G, I) and stained with tal1 (A, A′), lmo2 (B, B′), crim1 (C, C′), flk1 (D, D′), gdf6a/radar (E, E′), bmp4 (F, F′) and shh (G, G′). Arrows in A, A′, B′ and C′ denote the region of the intermediate cell mass (ICM). Note how the expression of these markers in the ICM is expanded in the crim1 morphants. It is also apparent that there are blood vessel defects as shown by the disorganised intersegmental vessels (Se) in D′ and major vessels in E′ (arrow). In pictures of the tail in G and G′, the inset (top right corner) shows the corresponding whole embryo. (H and H′). Whole mount immunohistochemistry of control-MO injected (G) and crim1A-MO injected (G′) embryos with En1 and visualized at 28 hpf. Embryos are shown laterally with anterior to the left. Se, intersegmental vessel; Scale bars=200 μm.

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Acknowledgments

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Reprinted from Mechanisms of Development, 123(4), Kinna, G., Kolle, G., Carter, A., Key, B., Lieschke, G.J., Perkins, A., and Little, M.H., Knockdown of zebrafish crim1 results in a bent tail phenotype with defects in somite and vascular development, 277-287, Copyright (2006) with permission from Elsevier. Full text @ Mech. Dev.