ZFIN Image: Kinna et al., 2006, Fig. 3

Image

Figure Caption

Fig. 3 Analysis of crim1 morphants. (A) Graph showing percentages of phenotypes scored for crim1A-MO injected embryos at decreasing concentrations. (B) Representative images for the scored phenotypes of crim1A-MO injected embryos at 24–30 hpf. The pig-tail phenotype is described as having a twisted tail. Severely affected embryos have a grossly expanded intermediate cell mass (ICM) as shown by the black arrow. Unlike the ‘severe’ and ‘pig-tail’ phenotypes, morphants with the mild phenotype will often survive past 48 hpf. (C–D′). Zebrafish embryos injected with 5 ng of Control morpholino (C, D), or crim1A-MO (C′, D′) and photographed at 36 hpf (C, C′) and 48 hpf (D, D′). Morphants that survive to hatching stage develop the characteristic hook-tail (arrow in D′). Scale bar=100 μm.

Acknowledgments

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Reprinted from Mechanisms of Development, 123(4), Kinna, G., Kolle, G., Carter, A., Key, B., Lieschke, G.J., Perkins, A., and Little, M.H., Knockdown of zebrafish crim1 results in a bent tail phenotype with defects in somite and vascular development, 277-287, Copyright (2006) with permission from Elsevier. Full text @ Mech. Dev.