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Fig. 7 Hsp90a and Steif/unc-45b mRNA are up-regulated in steif mutant embryos. In situ hybridization with antisense probes for hsp90a (A–B), a2 (C, D) and steif/unc-45 (E, F). steif mutant embryos (B, D, F) show an up-regulation of hsp90a, a2 and steif/unc-45b transcripts in skeletal muscles. Only hsp90a and a2 expression levels were increased in the heart (arrow and insert in panels A to D). Note that for reasons of quantitative comparisons, the staining reactions had to be terminated before strong signals were detectable in the wild-type embryos. All embryos are 2 days old. Scale bar: 150 mm.
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Reprinted from Developmental Biology, 308(1), Etard, C., Behra, M., Fischer, N., Hutcheson, D., Geisler, R., and Strähle, U., The UCS factor Steif/Unc-45b interacts with the heat shock protein Hsp90a during myofibrillogenesis, 133-143, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.