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Fig. 5 Steif/Unc-45b interacts with Hsp90a in vitro. (A–C) in situ hybridization with hsp90b (A), hsp90a (B) and hsp90a2 (C) antisense probes on 1-day-old embryos. hsp90a and a2 are strongly expressed in skeletal and heart muscles (arrow), while hsp90b is ubiquitously expressed. (D) in vitro pull-down assay. Steif-GST interacts with Hsp90a-GFP, Hsp90a2-GFP and Hsp90b-GFP but not GFP alone (lanes 1 to 4). GST without fused Steif does not pull down Hsp90a-GFP, Hsp90a2-GFP, Hsp90b-GFP or GFP (lanes 5 to 8). The Western blots were developed with anti-GFP antibody. M: protein standards indicated in kDa.
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Reprinted from Developmental Biology, 308(1), Etard, C., Behra, M., Fischer, N., Hutcheson, D., Geisler, R., and Strähle, U., The UCS factor Steif/Unc-45b interacts with the heat shock protein Hsp90a during myofibrillogenesis, 133-143, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.