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Fig. 3 charon expression in low-dose ntl morphants. A,B: Nine to 12 s. Dig-cha + DNP-ntl probes. Arrow: Notochord. Arrowhead: Site where cha is normally expressed. Developed for cha staining (left images, shown in dorsal view with tailbud to upper right) then redeveloped to show ntl staining (right images, shown in lateral view with tailbud to lower right). A: U: uninjected embryo. In right image, the ntl signal in the tail region obscures prior cha staining. B: Injected with 0.1 ng ntl-1 MO.
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