ZFIN Image: Schlueter et al., 2007, Fig. 2

Image

Figure Caption

Fig. 2 (A) Western immunoblot analysis of zebrafish lysates demonstrating overexpression of dominant-negative IGF1R:GFP fusion protein (dnIGF1R:GFP). First lane, lysates from zebrafish embryos injected with synthetic mRNA encoding GFP only; second lane, lysates from zebrafish embryos injected with synthetic mRNA encoding dnIGF1R:GFP. Upper band in dnIGF1R:GFP corresponds to the predicted molecular weight (∼60 kDa) of dnIGF1R:GFP after denaturation in reducing conditions. (B) Vasa immunostaining of PGCs (arrows) in zebrafish embryo overexpressing GFP only (control); (C) vasa immunostaining of PGCs in zebrafish embryo overexpressing dnIGF1R:GFP fusion protein. Magnification (B–C), 200x. Mean PGC numbers in panels B and C are summarized in Table 1.

Acknowledgments

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Reprinted from Developmental Biology, 305(1), Schlueter, P.J., Sang, X., Duan, C., and Wood, A.W., Insulin-like growth factor receptor 1b is required for zebrafish primordial germ cell migration and survival, 377-387, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.