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Fig. 3 santa negatively regulates vascular specific gene expression and endothelial cell morphology. (A–F) Bright-field micrographs of 60 hpf wild-type (A) and sans234 mutant (B) larvae. Black arrow in panel B points to enlarged heart and pericardial cavity. Endothelial cells visualized by in situ hybridization with tie2 in 60 hpf wild-type (C), and sans234 mutant (D) larvae, shown in ventral view of the heart region. Black bracket in panel D demarcates the expansion of tie2 expression in this region. Micrographs of cmlc2 (E) and tie2 (F) expression in 60 hpf san mutant larvae. The enlarged heart in sans234 mutant larvae shows an apparent increase of tie2 positive cells (black arrow in panel E and black bracket in panel F). (G–L) Epifluorescent micrographs of 36 hpf wild-type (G, I, and K) and san mutant (H, J, and L) Tg(tie2:GFP)s849 embryos showing the aortic arches (G and H), trunk vessels (I and J), and heart (K and L). Note the elevated Tg(tie2:GFP)s849 expression in sans234 mutant embryos (white arrows). (M–P) Epifluorescent micrographs of 60 hpf wild-type (M and O) and sans234 mutant (N and P) Tg(flk1:EGFP)s843 larvae showing the subintestinal vessel (M and N) and posterior trunk (O and P). Vessels in sans234 mutant larvae (white brackets) appear dilated compared to those in wild-type siblings. Abbreviations: BA: bulbus arteriosus, V: ventricle, A: atrium.
Reprinted from Developmental Biology, 307(1), Jin, S.W., Herzog, W., Santoro, M.M., Mitchell, T.S., Frantsve, J., Jungblut, B., Beis, D., Scott, I.C., D'Amico, L.A., Ober, E.A., Verkade, H., Field, H.A., Chi, N.C., Wehman, A.M., Baier, H., and Stainier, D.Y., A transgene-assisted genetic screen identifies essential regulators of vascular development in vertebrate embryos, 29-42, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.