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Fig. 8 Projection of pioneer axons is impaired in zebrafish ody mutant embryos. ody/ody;omp:yfp/+ (A-I) and omp:yfp/+ (J-L) embryos double labeled with zns-2 antibody (A,D,G,J; magenta in C,F,I,L) and anti-GFP antibody (B,E,H,K; green in C,F,I,L) at 1.5 dpf are shown in frontal views as z-stacked images. Projection patterns of pioneer axons in ody mutants vary, ranging from no axon reaching the presumptive OB (A-C) to an essentially normal projection (G-I). Note that projection defects with intermediate severity are observed (D-F). Arrowheads indicate accumulated zns-2-positive axons near the placode-telencephalon border. Scale bar: 50 µm. d, dorsal; l, lateral; op, olfactory placode; tel, telencephalon. The asterisk in E marks mispositioned olfactory neurons.