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Fig. 2 Application of 20 nM RA leads to widespread ectopic otic induction and to the formation of ectopic otic cells outside of the endogenous ear. (A) Application of 20 nM RA leads to ectopic pax8 expression encompassing the entire preplacodal domain (compare with Fig. 1J,L). (B-D) Embryos treated with 20 nM RA display ectopic patches of cells that express the otic marker stm and form enlarged vesicles. (C,D) Higher magnifications of B show the epithelial structure of these patches. Dorsal views with anterior towards the top. Scale bar: 35 µm for A; 50 µm for B; 10 µm for C,D.
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