|
Fig. S4 Mef2 knockdown reduces myhz1 but not tnnc mRNA in fast muscle. In situ mRNA hybridisation for myhz1 (A,B) and tnnc (C) (lateral view, anterior to left, dorsal to top). (A) Expression of myhz1 at 24 hpf in wild-type embryos injected with the indicated MOs. (B) myhz1 mRNA in wild type (top row) or embryos from a hoo heterozygote cross (bottom row) injected with mef2d MO. In wild type, mef2d MO had no effect, whereas <25% of the hoo lay appeared wild type, <50% mildly affected and <25% severely affected. (C) No change in level of tnnc in 24-hpf embryos injected with the indicated MO combinations.