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Fig. 5 Ncx4a is required for heart and gut LR patterning in zebrafish. (A-F) Expression pattern of the genes encoding Na,K-ATPase α2 and Ncx4a. Transcripts encoding Na,K-ATPase α2 (A) and Ncx4a (D) are detected prior to the mid-blastula stage. By the 10-somite stage, Na,K-ATPase α2 (B) and Ncx4a (E) are expressed in a broad range of tissues including Kupffer's vesicle (kv, arrow). By the 24-somite stage, Na,K-ATPase α2 is expressed predominantly in the somites (C), whereas Ncx4a remains ubiquitous (F). (G) Bar chart of the percentage of embryos exhibiting heart and/or gut laterality defects.
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