ZFIN Image: Draper et al., 2007, Fig. 2

Image

Figure Caption

Fig. 2 The nos1(fh49) mutant allele encodes a truncated form of the protein. (A) Sequence traces of nos1 from wild-type (top) and nos1(fh49) heterozygotes reveal that nos1(fh49) is a point mutation that converts the Leu(110) codon into a stop codon. The mutation disrupts an MseI restriction enzyme site present in wild-type. (B) Schematic of the predicted wild-type and mutant Nos1 protein structures. The CCHC zinc finger RNA-binding domains are shown in yellow. (C) PCR-based genotyping assay utilizing the MseI restriction site polymorphism. nos1(fh49) heterozygotes have both wild-type (lower) and mutant (upper) bands.

Acknowledgments

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Reprinted from Developmental Biology, 305(2), Draper, B.W., McCallum, C.M., and Moens, C.B., nanos1 is required to maintain oocyte production in adult zebrafish, 589-598, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.