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Fig. 3 Gene comparison, expression and colocalization of Mib2 and Mib. (A) Comparison of Mib2 proteins. The percentage of amino acid identity of different domains of Mib2 from different species (z: zebrafish; F: Fugu; h: human; m: mouse; D: Drosophila) and zMib compared to those of zMib2 is given. (B) RT-PCR analysis of mib2 expression. cyclin b mRNA is amplified as a control. mib2, like mib, is ubiquitously expressed. (C) Mib and Mib2 are colocalized in COS7 cells.
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Reprinted from Developmental Biology, 305(1), Zhang, C., Li, Q., Lim, C.H., Qiu, X., and Jiang, Y.J., The characterization of zebrafish antimorphic mib alleles reveals that Mib and Mind bomb-2 (Mib2) function redundantly, 14-27, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.