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Fig. 3 Maternal expression of zebrafish ror genes. (A–C) Whole-mount in situ hybridization using rora, b, and c riboprobes on 1-cell stage embryos reveal expression only for rorc. Lateral view, animal pole to top. Scale bar: 200 μm. (D) Semi-quantitative RT-PCR using cDNA samples prepared from unfertilized eggs (0 hpf) and early embryos (1–6 hpf) confirms that rorc exists as a maternal transcript.
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Reprinted from Gene expression patterns : GEP, 7(5), Flores, M.V., Hall, C., Jury, A., Crosier, K., and Crosier, P., The zebrafish retinoid-related orphan receptor (ror) gene family, 535-543, Copyright (2007) with permission from Elsevier. Full text @ Gene Expr. Patterns