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Fig. 4 fgf1 morphants have persistent high levels of gata1 expression at 35 hours postfertilization (hpf). The 35 polymerase chain reaction (PCR) cycles with gata1-specific primers amplify visibly less product from control morphants than from fgf1 morphants at 35 hpf (standard reverse transcriptase-quantitative PCR). Comparison to 24 hpf control and fgf1 morphants indicates continuously high gata1 expression in fgf1 morphants, but a down-regulation in control morphants, confirming the in situ hybridization data. bactin2 (beta actin2) expression is used as control. Identical results were observed in three independent sets of experiments.