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Fig. 3 Sequential immunoprecipitations to investigate Ldb1- and Eto-2-containing complexes. (A) Nuclear extracts of noninduced normal C88 cells were first depleted of either Ldb1 or Eto-2 with their respective antibodies. The supernatants were then incubated with α-Eto-2 or α-Ldb1, respectively. The second supernatant was also loaded to determine which proteins do not interact with either Eto-2 or Ldb1. IgG lanes are control immunoprecipitations carried out with a nonspecific, isotype-matched antibody. (B) Scheme of interacting factors from IP experiments in A (see also Fig. S1A in the supplementary material). Each new line represents the analysis of an additional three transcription factors.