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Fig. 2 Expression of zebrafish smc3 during embryogenesis. A) Semiquantitative RT-PCR analysis of smc3 transcript level in zebrafish embryos at different times during development. ef-1 transcript level, which is expressed throughout development, was used as internal control. B) Expression of constituents of the cohesin multimeric protein complex: zebrafish smc1, rad21 and sa2 transcripts were quantitated as described in panel A. C) Spatio-temporal expression of smc3 mRNA. Whole-mount mRNA in-situ hybridization was performed at the indicated developmental stages. Left-to-right: lateral view with the animal pole up of embryos at 50% epiboly (6 hpf), at 90% epiboly (9 hpf), at 3-somite stage (12 hpf), and at 24 hpf.