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Fig. 7 The Wdr68 protein co-localizes with Dyrk1a. Transiently transfected HEK-293FT cells. A, E, I) Fluorescence of GFP and fusion proteins. B, F, J) Fluorescence of mRFP1 and fusion proteins. C, G, K) Fluorescence of nuclei stained with DAPI. D, H, L) Composite overlay image of GFP, RFP and DAPI signals. A, B, C, D) GFP and mRFP1 distribute throughout the cytoplasm and nucleus. E, F, G, H) The GFP-Wdr68 fusion and the mRFP1-Dyrk1a fusion are co-localized to the nucleus. I, J, K, L) The GFP-T284F Wdr68 mutant does not co-localize with nuclear mRFP1-Dyrk1a.
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