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Fig. 7 Targets of Fgf signaling investigated by SU5402 treatment, with DMSO control. (A,F) Alcian green staining reveals a loss of oral teeth (arrowhead and inset in A) following SU5402 treatment. Arrowhead indicates dentary bone in F. (B,C,G,H) Mesenchymal (arrow), but not epithelial (arrowhead), expression of Astyanax pax9 is absent after SU5402 treatment. Mesenchymal expression of lhx6 (D,I, arrows), epithelial expression of fgf8 (E,J, arrowheads) and dlx2b (L,Q, arrowheads), and both mesenchymal (arrow) and epithelial (arrowhead) expression of dlx2a (K,P) in the oral region are absent after SU5402 treatment in Astyanax. (M,N,R,S) Oral epithelial expression of shh and pitx2 remains after SU5402 treatment in Astyanax. (O,T) Mandibular arch mesenchymal expression of dlx2a (arrow) in the zebrafish is absent after SU5402 treatment. (B,D-E,G,I-K,M-P,R-T) Whole-mount ventral views; (A,F,L,Q) lateral views; (C,H) transverse sections. Abbreviations as in Fig. 1. a, adhesive gland; f, forebrain; pq, palatoquadrate; pt, pharyngeal tooth. Scale bars: 100 μm.