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Fig. 9 Scn8aa expression is restricted to the nervous system. A-E: At 19 hours postfertilization (hpf), scn8aa mRNA is detected in the developing trigeminal ganglion and spinal cord. A,B: In anterior portions of the embryo, scn8aa mRNA is detected in the developing trigeminal ganglion (tg). C: In the spinal cord, scn8aa transcripts are detected dorsally in Rohon-Beard (RB) cells as well as ventrally (asterisk). D: Under the same conditions, the sense probe does not reveal a signal. E-H: At 24 hpf, scn8aa expression expands within the hindbrain. E,F: scn8aa transcripts are detected in ventral hindbrain at 24 hpf (arrowheads) and the trigeminal ganglion. G: In the spinal cord, scn8aa expression occurs in RB cells and diffusely in ventral regions. H: The sense probe does not reveal a signal at 24 hpf. I-L: At 60 hpf, scn8aa expression appears diffusely in both rostral and caudal regions of the central nervous system. I,J: In rostral regions of the central nervous system, scn8aa expression is detected in the retina and diffusely in the brain. K: In the spinal cord, expression is detected dorsally in isolated cells (dots) and more diffusely in ventral regions (asterisks). In addition, expression is detected in the periphery, in dorsal root ganglia (arrows). L: The sense probe did not reveal a signal at 60 hpf. as, antisense probe; n, notochord; ot, otic vesicle; ret, retina; s, sense probe; y, yolk sac; yse, yolk sac extension. Scale bars = 250 μm in A (applies to A,E,I), 100 μm in B (applies to B,F,J), 100 μm in C (applies to C,D,G,H,K,L).