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Fig. S1 Overexpression of 150 pg shh-mRNA leads to an enlargement of the thalamic domain of neurog1 compared with wild type at 28 hpf (A,A¢, width indicated by yellow arrows). The thalamic expression of neurog1 is absent in smu mutant embryos at 32 hpf (B,B¢, yellow arrow marks the thalamus). In the presumptive ZLI, shh expression is detectable in oep mutant embryos at 20 somites first (C,C¢, marked by black arrow).
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