|
Fig. 9 Midline defects in oep flh and oep ntl double mutants. (A-L) Expression of shh in wild-type (A,B), oep mutant (C,D), flh mutant (E,F), ntl mutant (I,J), oep flh double mutant (G,H) and oep ntl double mutant (K,L) embryos at the 11-12 somites stage. (A,C,E,G,I,K) lateral view; (B,D,F,H,J,L) dorsal view of anterior trunk region. (A,B) Expression of shh in ventral neuroectoderm including ventral diencephalon and floor plate (arrowhead), and notochord in wild-type embryos. (C,D) Expression of shh in notochord and few floor plate cells (arrowhead) of oep mutants. (E,F) Expression of shh in ventral neuroectoderm, anterior floor plate and scattered posterior floor plate cells (arrowhead) in flh mutants. (G,H) Absence of shh expression in oep flh double mutants. (I,J) Expression of shh in the midline in presumptive floor plate cells (arrowhead) of ntl mutants. (K,L) Expression of shh in scattered cells in the anterior trunk (arrowhead) of oep ntl double mutants.