ZFIN Image: Halpern et al., 1997, Fig. 8

Image

Figure Caption

Fig. 8 Floor plate but not notochord is present in cyc;ntl double mutants. Left side views, with anterior to the left and dorsal to the top of whole-mounted 24-hr embryos. RNA in situ hybridization with a col2a1 probe (Yan et al., 1995). In the wild-type (A) expression is in the notochord (dark arrowhead), in the single row of cells that comprise the floor plate (white arrowhead), and in the hypochord (hy). The floor plate is specifically missing in cyc^- (B). The notochord and possibly hypochord (Rissi et al., 1995) are missing in ntl^- (C), whereas the floor plate is present (arrowhead), as are col2a1-expressing cells just ventral to the spinal cord and floorplate (arrow). Floor plate is also present in the cyc;ntl double mutant hindbrain and trunk spinal cord (D) but labeling does not extend as far caudal as in ntl mutants. Cells beneath the floor plate also label in double mutants but these are fewer in number than in ntl single mutants (arrow, compare with C). Scale bar, 25 um.

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Reprinted from Developmental Biology, volume 187(2), Halpern, M.E., Hatta, K., Amacher, S.L., Talbot, W.S., Yan, Y.-L., Thisse, B., Thisse, C., Postlethwait, J.H., and Kimmel, C.B., Genetic interactions in zebrafish midline development, 154-170, Copyright 1997, with permission from Elsevier.

Reprinted from Developmental Biology, 187(2), Halpern, M.E., Hatta, K., Amacher, S.L., Talbot, W.S., Yan, Y.-L., Thisse, B., Thisse, C., Postlethwait, J.H., and Kimmel, C.B., Genetic interactions in zebrafish midline development, 154-170, Copyright (1997) with permission from Elsevier. Full text @ Dev. Biol.