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Fig. 4 Expression of Ntl protein in ntl mutant embryos. (A-D) Immunostaining of wild-type (A,C), ntlb160 (B), and ntlb195 (D) embryos. (A,B) Vegetal pole view of 95% epiboly embryos. (C,D) Posterior trunk and tail region of 20-hour old embryos. Ntl protein was not detectable in whole-mount antibody preparations of mutant embryos.
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