ZFIN Image: Jin et al., 2006, Fig. 2

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Figure Caption

Fig. 2 The enhanced green fluorescence protein (EGFP) protein expression pattern in the Tg(5' 5kbscl:EGFP)^a transgenic fish. A-H: Lateral (A-C,G,H) and dorsal (D-F) views of the 6-somite (6s), 12-somite (12s), 18-somite (18s), 24 hours postfertilization (hpf), and 48 hpf stage Tg(5' 5kbscl:EGFP)^a embryos, respectively, show the EGFP protein expression in a manner similar to the endogenous Scl protein expression pattern. I,J: Lateral views of 18-somite stage (18s) Tg(5' 5kbscl:EGFP)^a embryos immunohistochemistry stained with anti-EGFP (I, green) and anti-zebrafish Scl (J, red) antibodies. K: Superimposed view of I and J (original magnification, x10). I'-K' Confocal images (x100 magnification) of the boxed region in I, J, and K confirm the colocalization of the EGFP protein (green) with the endogenous Scl protein (red). In all the panels, embryos are oriented with anterior to the left.

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Expression / Labeling details

Acknowledgments

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