ZFIN Image: Lamason et al., 2005, Fig. 2

Image

Figure Caption

Fig. 2 Rescue and morpholino knockdown establish slc24a5 as the golden gene. Lateral views of 48-hpf (A) wild-type and (B) gol^b¹ zebrafish larvae. (C) 48-hpf wild-type larva injected with morpholino targeted to the translational start site of slc24a5 phenocopies the gol^b¹ mutation. Lateral view of eye (D) and dorsal view of head (E) of 72-hpf wild-type embryos. (F and G) gol^b¹ pigmentation pattern at 72 hpf, showing lightly pigmented cells. (H and I) 72 hpf gol^b¹ larva injected with PAC215f11 show mosaic rescue; arrow identifies a heavily pigmented melanophore. (J and K) 72-hpf gol^b¹ larva injected with full-length zebrafish slc24a5 RNA. (L and M) 72-hpf gol^b¹ larvae injected with full-length human European (Thr¹¹¹) SLC24A5 RNA. Rescue with the ancestral human allele (Ala¹¹¹) is shown in fig. S4. Rescue in RNA-injected embryos is more apparent in melanophores (K) and (M) than in RPE. Scale bars in [(A) to (C)], 300 µm; in (D), (F), (H), (J), and (L), 100 µm; in (E), (G), (I), (K), and (M), 200 µm.

Acknowledgments

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Excerpted with permission from Lamason et al., Science 310(5755):1782-1786. Copyright 2005 AAAS. "Readers may view, browse, and/or download material for temporary copying purposes only, provided these uses are for noncommercial personal purposes. Except as provided by law, this material may not be further reproduced, distributed, transmitted, modified, adapted, performed, displayed, published, or sold in whole or in part, without prior written permission from the publisher."

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